The Invisorb® Spin Tissue Mini Kit simplifies genomic DNA purification from a variety of tissue types (e.g. muscle, liver, heart, brain, mouse tail or insects) and cells. PCR inhibitors, which can interfere with the amplification reaction, are efficiently removed. Pure DNA is eluted in 50 – 200 µl EDTA-free buffer or water and can be used in subsequent downstream applications, such as PCR, real-time PCR, SNP detection, Southern Blotting, sequencing and cloning.
- Optimized protocols for a variety of starting materials, complete removal of contaminants and inhibitors
- More intact DNA: no DNA degradation by using a gentle, low-salt (non-chaotropic) buffer system
- For In Vitro Diagnostic Use (CE-IVD)*
Starting Material Average Yield Preparation Time 0.5 - 40 mg tissue
1.2 cm rodent tails
10 –106 eukaryotic cells
up to 50 μg DNA from tissue
up to 10 μg DNA from cells
15 min after lysis
The Invisorb Spin Tissue Mini Kit uses a special buffer and Proteinase S for efficient lysis of the tissue samples. After a centrifugation step the precleaned lysate is mixed with the binding buffer and transferred onto the spin filter column. The DNA binds to the membrane, impurities are washed away and reproducible ultra-pure DNA is eluted. The purified DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:
- real-time PCR
- RFLP analysis
- restriction enzyme digestion
- SNP detection
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized
1. Higher DNA yields using Proteinase S
DNA was isolated using Proteinase S and Proteinase K from 30 mg of rat liver. For both protocols the DNA was eluted in 200 µl Elution Buffer and 5 µl of the eluates were analyzed on a 0.8 % agarose gel stained with ethidium bromide. The Proteinase S treatment resulted in higher DNA yields. (Marker - GeneRuler™ DNA Ladder, Fermentas)
2. More intact DNA
Equal amounts of rat tissues were used to isolate DNA using the Invisorb Spin Tissue Mini Kit (I, non-chaotropic buffers). For comparison with chaotropic chemistry an equivalent kit from a major supplier (c) was evaluated. Standard protocols for both kits were used with comparable conditions for Proteinase K treatment. For both kits the DNA was eluted in 200 µl Elution Buffer and 5 µl of the eluates were analyzed on a 0.8 % agarose gel stained with ethidium bromide.
3. Sex determination of birds
DNA was isolated with the Invisorb Spin Tissue Mini Kit from quill for sex determination of birds; and 1/10 of the eluted DNA was amplified and analyzed on a 2 % agarose gel.
Lane 1+4: 100 bp ladder
Lane 2: no fragment appears, no femal bird
Lane 3: 1 fragment appears: identifies the bird as a male one
Data kindly provided from Dr. M. Kuhn, Congen GmbH, Berlin
4. Detection of points mutations in the tumor suppresor gene p16 by SSCP-PCR
DNA was isolated from paraffin embedded tissue of 4 different samples with Invisorb Spin Tissue Mini Kit and 1/10 of the eluted DNA was analyzed in a nondenatured 10 % PAA gel by silver staining:
Lane 1: negative control
Lane 2,3: sample 1
Lane 4,5: sample 2
Lane 6,7: sample 3
Lane 8,9: sample 4
For each sample DNA was isolated from tumor tissue (lane 2,4,6,8) and normal tissue (lane 3,5,7,9).
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Phenotypic polymorphism of Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) may lead to species misidentification.
Grella MD, Savino AG, Paulo DF, Mendes FM, Azeredo-Espin AM, Queiroz MM, Thyssen PJ, Linhares AX
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Genetic Diversity and Structure of Brazilian Populations of Diatraea saccharalis (Lepidoptera: Crambidae): Implications for Pest Management.
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Lange C, Brunswig-Spickenheier B, Cappallo-Obermann H, Eggert K, Gehling UM, Rudolph C, Schlegelberger B, Cornils K, Zustin J, Spiess AN, Zander AR
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Campylobacter fetus subspecies fetus spondylodiscitis.
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Limited immune-modulating activity of porcine mesenchymal stromal cells abolishes their protective efficacy in acute kidney injury.
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Fumiere O, Marien A, Fernandez Pierna JA, Baeten V, Berben G
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Pujadas L, Gruart A, Bosch C, Delgado L, Teixeira CM, Rossi D, de Lecea L, Martínez A, Delgado-García JM, Soriano E
J Neurosci. 2010 Mar 31;30(13):4636-49
Sudden infant death syndrome and sudden intrauterine unexplained death: correlation between hypoplasia of raphé nuclei and serotonin transporter gene promoter polymorphism.
Lavezzi AM, Casale V, Oneda R, Weese-Mayer DE, Matturri L
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Psittacid herpesvirus DNA in a pancreatic duct carcinoma in a macaw.
Mundhenk L, Müller K, Lierz M, Lüschow D, Stahl T, Müller H, Johne R
Vet Rec. 2009 Mar 7;164(10):306-8
Genetic determination of sibship and twin zygosity in a case of an alleged double infant homicide.
von Wurmb-Schwark N, Schwark T
Leg Med (Tokyo). 2009 Apr;11 Suppl 1:S510-1
Subtelomeric DNA hypomethylation is not required for telomeric sister chromatid exchanges in ALT cells.
Tilman G, Loriot A, Van Beneden A, Arnoult N, Londoño-Vallejo JA, De Smet C, Decottignies A
Oncogene. 2009 Apr 9;28(14):1682-93
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Cindoruk M, Cirak MY, Unal S, Karakan T, Erkan G, Engin D, Dumlu S, Turet S
Eur J Gastroenterol Hepatol. 2008 Jan;20(1):33-6
Loss of heterozygosity and copy number abnormality in clear cell renal cell carcinoma discovered by high-density affymetrix 10K single nucleotide polymorphism mapping array.
Toma MI, Grosser M, Herr A, Aust DE, Meye A, Hoefling C, Fuessel S, Wuttig D, Wirth MP, Baretton GB
Neoplasia. 2008 Jul;10(7):634-42
The potential for beta-structure in the repeat domain of tau protein determines aggregation, synaptic decay, neuronal loss, and coassembly with endogenous Tau in inducible mouse models of tauopathy.
Mocanu MM, Nissen A, Eckermann K, Khlistunova I, Biernat J, Drexler D, Petrova O, Schönig K, Bujard H, Mandelkow E, Zhou L, Rune G, Mandelkow EM
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|Product||Package size||Catalogue No|
|Invisorb Spin Tissue Mini Kit||250 purifications||1032100300|