The Invisorb® Spin Blood Mini Kit is the ideal tool for fast and convenient purification of genomic DNA from human and animal blood as well as from buffy coat. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used. Prior separation of leukocytes is not necessary.
- Fast & safe handling: no additional erythrocyte lysis , capped spin columns
- Flexible system: compatible with a variety of anticoagulants and tubes
- For In Vitro Diagnostic Use (CE-IVD)*
Starting Material Average Yield Preparation Time up to 200 µl human or animal blood
up to 30 µl buffy coat
up to 10 µg (in average 6 µg), depending on amount of lymphocytes, sample source, transport and storage about 25 min
The whole blood sample is lysed in an optimized lysis buffer and proteins are degraded during the lysis with Proteinase S. The DNA binds to filter membrane, followed by washing steps and the final elution. The procedure requires minimal interaction by the user, allowing safe handling of potentially infectious samples. The purified genomic DNA is of high quality and can be used for subsequent downstream applications:
- real-time PCR
- RFLP analysis
- restriction enzyme digestion
- SNP analysis
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
1. Higher DNA yields using Proteinase S
DNA was isolated using Proteinase S and Proteinase K from 200 µl of whole blood. For both protocols the DNA was eluted in 200 µl Elution Buffer and 5 µl of the eluates were analyzed on a 0.8 % agarose gel stained with ethidium bromide. The Proteinase S treatment resulted in higher DNA yields. (Marker - GeneRuler™ DNA Ladder, Fermentas)
2. Influence of different blood collection systems on DNA recovery
Genomic DNA was isolated from 100 µl of the same blood sample, stabilized with different anticoagulants: EDTA and citrate. Six different blood collection systems were used (see table). 10 µl of the DNA eluates were loaded onto a 0.8 % agarose gel, stained with ethidium bromide. (Marker - GeneRuler™ DNA Ladder, Fermentas)
3. Isolation of DNA from various blood samples with different storage conditions
DNA was isolated from 5 different 200 μl blood samples using the Invisorb Spin Blood Mini Kit, eluted in 200 μl of Elution Buffer. 10 μl of the eluate was loaded on 0.8 % agarose gel, ethidium bromide stained.
lanes 1+2 fresh human blood
lanes 3+4 4 °C stored human blood
lanes 5+6 frozen human blood
lanes 7+8 frozen / thawed human blood (5x)
lanes 9+10 cattle blood, 4 years old
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|Product||Package size||Catalogue No|
|Invisorb Spin Blood Mini Kit||250 purifications||1031100300|