The Invisorb® Fragment CleanUp provides a convenient tool for fast and efficient purification of PCR products and DNA fragments from amplification or enzymatic reactions as well as for fast and efficient extraction of DNA fragments from agarose gels.
- PCR cleanup ready in 7 minutes - only binding and elution
- Elution volume as little as 10 μl, also suitable for sample concentration
- Dye removal from Cycle-Sequencing reactions
- Multiple applications in one kit incl. gel extraction
Starting Material Average Yield Preparation Time up to 100 ml PCR-, Cycle Sequencing- cDNA synthesis-, ligation- and restriction digestion reaction mixture
up to 300 mg gel slices from TAE or TBE agarose gels
up to 95 %, depending on fragment
length and kind of agarose gel
7 min for PCR cleanup
20 min for gel extraction
For PCR or DNA fragment cleanup the MSB technology is used, i.e. washing steps are not necessary. After adjustment of binding conditions the DNA fragments bind to the membrane of the spin filter. The flow-through contains all contaminants. Finally, the DNA fragments are eluted in a low-salt buffer or water. For purification of DNA fragments from agarose gels, the DNA fragments are bound to the surface of the spin filter column after the gel is solubilized. The purified DNA fragments are ready to use in various downstream applications:
- digestion with restriction enzymes
High and reproducible recovery with low elution volumes
DNA fragments of different sizes (GeneRuler™ DNA Ladder, Fermentas) were purified according to the standard protocol using different elution volumes as shown. For analysis the DNA was loaded onto a 1% TAE agarose gel.
(A) Per lane the same DNA amount was loaded onto the gel (2,4,6,8 and 10 µl) to show absolute yield and the reproducibility.
(B) 5 µl of each eluate were loaded onto the gel to show the concentration.
User manual download
Genetic Diversity and Structure of Brazilian Populations of Diatraea saccharalis (Lepidoptera: Crambidae): Implications for Pest Management.
Silva-Brandão KL, Santos TV, Cônsoli FL, Omoto C
J Econ Entomol. 2015 Feb;108(1):307-16
The Enterobacterium Trabulsiella odontotermitis Presents Novel Adaptations Related to Its Association with Fungus-Growing Termites.
Sapountzis P, Gruntjes T, Otani S, Estevez J, da Costa RR, Plunkett G 3rd, Perna NT, Poulsen M
Appl Environ Microbiol. 2015 Oct 1;81(19):6577-88
The etiological agent of cotton ramulosis represents a single phylogenetic lineage within the Colletotrichum gloeosporioides species complex.
Salustiano, Maria Eloisa, Rondon, Marina Nunes, Abreu, Lucas M., Costa, Sarah da Silva, Machado, José da Cruz, & Pfenning, Ludwig H
(2014).Tropical Plant Pathology, 39(5), 357-367
Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes.
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Mem Inst Oswaldo Cruz. 2014 Jul;109(4):428-35
Lasting influence of biochemically contrasting organic inputs on abundance and community structure of total and proteolytic bacteria in tropical soils.
Frank Rasche, Mary K. Musyoki, Carolin Röhl, Esther K. Muema, Bernard Vanlauwe, Georg Cadisch
Soil Biology and Biochemistry, Volume 74, Issue null, Pages 204-213
Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae).
Emadzade K, Jang TS, Macas J, Kovařík A, Novák P, Parker J, Weiss-Schneeweiss H
Ann Bot. 2014 Aug 28. pii: mcu178
Antifungal susceptibilities and identification of species of the Sporothrix schenckii complex isolated in Brazil.
Ottonelli Stopiglia CD, Magagnin CM, Castrillón MR, Mendes SD, Heidrich D, Valente P, Scroferneker ML
Med Mycol. 2014 Jan;52(1):56-64
Comparative evaluation of the gut microbiota associated with the below- and above-ground life stages (larvae and beetles) of the forest cockchafer, Melolontha hippocastani.
Arias-Cordero E, Ping L, Reichwald K, Delb H, Platzer M, Boland W
PLoS One. 2012;7(12):e51557
Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-cresol-degradative strains of Pseudomonas fluorescens.
Jõesaar M, Heinaru E, Viggor S, Vedler E, Heinaru A
FEMS Microbiol Ecol. 2010 Jun;72(3):464-75
Study of the molecular mechanisms involved in high-level macrolide resistance of Spanish Campylobacter jejuni and Campylobacter coli strains.
Pérez-Boto D, López-Portolés JA, Simón C, Valdezate S, Echeita MA
J Antimicrob Chemother. 2010 Oct;65(10):2083-8
Simultaneous presence of bovine papillomavirus in blood and in short-term lymphocyte cultures from dairy cattle in Pernambuco, Brazil.
Diniz N, Melo TC, Santos JF, Mori E, Brandão PE, Richtzenhain LJ, Freitas AC, Beçak W, Carvalho RF, Stocco RC
Genet Mol Res. 2009 Dec 15;8(4):1474-80
|Product||Package size||Catalogue No|
|Invisorb Fragment CleanUp||250 purifications||1020300300|